General description Acetonitrile (MeCN) is widely employed as a solvent in various studies and has high dielectric constant (37.5). In the early days of HPLC, THF was commonly used as a mobile phase solvent. Our samples are diluted with a mixture of acetonitrile / water (50/50). If another grade such as ACS grade acetonitrile was used your sample would contaminants and peaks and valleys all over the place giving you a false identification. It is used as a polar aprotic solvent … Acetonitrile together with Methanol are the most commonly used solvents for HPLC and UHPLC Chromatography processes because most substances are easily soluble in both solvents, but not saturated hydrocarbons. Acetonitrile has a low wavelength which means that the solvent will elute first … Acetonitrile, often abbreviated MeCN (methyl cyanide), is the chemical compound with the formula CH 3 CN.This colourless liquid is the simplest organic nitrile (hydrogen cyanide is a simpler nitrile, but the cyanide anion is not classed as organic).It is produced mainly as a byproduct of acrylonitrile manufacture. We will be discussing the solvents used in the HPLC mobile phase as their selection determine how the analytes can be separated. the solvents are used … It is imperative that your solvents are not only clean but also reliable. Methanol is a very common choice for the HPLC mobile phase organic solvent component in reversed phase HPLC even though acetonitrile is often superior in several aspects. The organic solvents acetonitrile and methanol are often used as the mobile phase in reversed-phase chromatography. This is why only high purity HPLC grade acetonitrile can be used. It has eluting strength similar to acetonitrile, but just slightly stronger. Acetonitrile (MeCN) and methanol (MeOH) are the most commonly used organic modifiers in reversed-phase chromatography. These can only be used with APCI and not electrospray which needs a polar mobile phase for ionisation. The solvents used in the HPLC mobile phase are chosen on the basis of the type of HPLC selected for the purpose. There are two main types of HPLC used: Normal Phase Chromatography - separation is accomplished by exploiting analytes varying affinity for a polar stationary phase and uses non-polar solvents such as Chloroform, Hexanes, cyclohexane, etc. These characteristics allow TLC separation data to be more effectively transferred to flash chromatography and allow the use of higher strong solvent (acetonitrile) percentages in the gradient. Since it technically is an ether and is very miscible with water, it is sometimes useful with reverse phase HPLC. Commercial prices of these solvents are relatively expensive, particularly Acetonitrile for HPLC. Reversed-Phase Solvent Choices in Order of Increasing Strength • Mobile phase without buffer salts • 100% Methanol • 100% Acetonitrile • 75% Acetonitrile:25% Isopropanol • 100% Isopropanol • 100% … Hello Agnes, we use acetonitrile and water (with modifier 0.1% TFA or 0.1% NH3) as solvents. The benefits you can realize from using acetonitrile … Although both solvents offer certain advantages and disadvantages, one of their key strengths, from a chromatographic perspective, is that they offer substantially different selectivity, and as such, are … On the other hand, methanol is often less expensive and less toxic than acetonitrile, so it has advantages as well. Solvents • Normal phase solvent Normal phase solvents such as dichloromethane, hexane and toluene have been used but not regularly than reverse phase solvents. 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